请使用支持JavaScript的浏览器!
主营:核苷酸,miRNA抑制剂,PNA探针和Fmoc单夹
banner
当前位置: 首页 > 产品中心 > > Pnabio/./Liquid form 5 mg/ml, 3000 pmol(30 uM x 20 ul x 5 tubes)/Cas9 protein with NLS, Liquid form, 500 ug (100 ug x 5)/CP04-500/CP04-500

Pnabio/./Liquid form 5 mg/ml, 3000 pmol(30 uM x 20 ul x 5 tubes)/Cas9 protein with NLS, Liquid form, 500 ug (100 ug x 5)/CP04-500/CP04-500

Pnabio/./Liquid form 5 mg/ml, 3000 pmol(30 uM x 20 ul x 5 tubes)/Cas9 protein with NLS, Liquid form, 500 ug (100 ug x 5)/CP04-500/CP04-500
  • Pnabio/./Liquid form 5 mg/ml, 3000 pmol(30 uM x 20 ul x 5 tubes)/Cas9 protein with NLS, Liquid form, 500 ug (100 ug x 5)/CP04-500/CP04-500
商品介绍

Cas9protein,incomplexwithguideRNA,offersbenefitforCRISPR/Cas9technologyoverplasmidormRNAtransfection.ThebiggestbenefitisthatyoucanmakeRNPcomplexthatisreadytoworkinvivo.Cas9proteinisshortlived(about24hoursofhalflifeincells),thusminimizetheoff-targeteffectsandmosaicism.YoucanalsoeasilytesttheactivityofguideRNAinvitrobyasimplecleavageassay.Inaddition,Cas9proteinisprettystable,andactivityconfirmationismucheasiercomparedtomRNA.Lastly,itisactiveinmorebroadrangeoforganismssuchasnematodes,insects,plants,andnon-typicalmodelsystemsasitdoesnotrequiretranscriptionortranslation.

Cas9proteinofferedbyPNABioisconjugatedwithNLS,anditisinjection/transfectionreadyform.

Cy3-Cas9canbeaneasyenrichmentofthecellsthataretransfectedwithCRISPR/Cas9complex.

Invitrocleavageassay

Cas9 protein NLS cleavage assay with sgRNA

PCRproductwasincubatedwithvariousamountsofCas9proteinandrunonthegel.InvitrocleavageassayisasimplewaytochecktheactivityofsgRNA.

TransfectionofRNPcomplex

Cas9 protein NLS cleavage assay with sgRNA

RNPcomplexofsgRNAandvariousamountofCas9proteinwastransfectedintoNIH3T3cellsbyelectropration(Neon)orbylipofectamine(LF2000).

InjectionofRNPcomplex

Cas9 protein NLS cleavage assay with sgRNA

Formouseembryoinjection,RNPcomplexof60ng/ulofCas9proteinand15ng/uleachoftwosgRNAswasinjectedintomouseembryo.ThetailDNAwaspreppedfromthepupsandsubjecttoT7E1assay.GreyrepresentsWT,greenforlargedeletion(#2,3,4,8),redforsmallin/del(#5,6,7),blueforhomozygoussmallin/del(#9,10).

品牌介绍

Pnabio


PNA生物提供优质的PNA(肽核酸)产品,包括定制的寡糖,FISH探针,miRNA抑制剂,PNA钳夹探针和FMOC单体。


PNA BIO INC is devoted to provide quality research products and services to the laboratories of basic research and applied field.


PNA Bio offer quality PNA (Peptide Nucleic Acid) products including custom oligos, FISH probes, miRNA inhibitors, PNA clamp probes and Fmoc monomers. They are provided >95% purity after HPLC purification along with MS/MS data. PNA products are manufactured by Panagene.


PNA Bio also provides engineered nuclease service including CRISPR/Cas9 nucleases (RGEN) synthesis, validation and Cas9 protein in addition to knock-out and knock-in cell line services. RGEN and related products are manufactured by ToolGen Inc.


品牌

货号

名称

规格

Pnabio

F1001

TelC telomere probe

1

Pnabio

CP01

Cas9 protein with NLS (injection ready);reconstitutes to 1 mg/ml (6 uM)

250 ug (1530 pmol / 30 uM x 50 ul)

Pnabio

CP02

Cas9 protein with NLS (injection ready); reconstitutes to 5 mg/ml (30 uM);

1 mg (250 ug x 4)

Pnabio

CP03

Cas9 protein with NLS (injection ready); reconstitutes to 5 mg/ml (30 uM);

100 ug (20 ug x 5)

Pnabio

CP04-100

Cas9 protein with NLS (injection ready); Liquid form, 1 mg/ml (6 uM);

500 ug (100 ug x 5)

Pnabio

CP04-500

Cas9 protein with NLS (injection ready); Liquid form, 5 mg/ml (30 uM);

50 ug


自营商城图标
厂家直采
全球直采模式 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔
  • 服务热线:
    4000-520-616

    固话:
    0512-67156496

    手机:
    18915418616

    邮箱:
    info@ebiomall.com
    工作时间:
    9:00 - 18:00
  • -平行进口 原厂直采-

    立即搜索

    我们与欧美近百家中小品牌签订代理,近千家海外生物有合作,可以帮忙代购,免费询价.您也可以加入我们QQ群[616054153]
    或者扫上面微信[ebiomall08],加我好友,备注:进群,我们会第一时间拉你进销售咨询群
  • 收起>>